#18 · Genomics
Chromosome-scale and draft genomes of polyploid and non-model eukaryotes
The shared thread is the construction of contiguous reference genomes for polyploid and non-model eukaryotes from long-read and proximity-ligation data. Two preprints report chromosome-scale assemblies of durum wheat landraces Chili and Mahmoudi from HiFi and Hi-C reads, each resolved into 14 pseudomolecules. A third preprint presents a PacBio HiFi draft of Greyia radlkoferi, a 206 Mb assembly with scaffold N50 of 10.1 Mb and 17,804 predicted protein-coding genes, at draft rather than chromosome-scale resolution. The published study on sugarcane extends this logic into functional genetics: a phased POJ2878 genome built with Pore-C is paired with polyploid-tailored GWAS across 981 accessions linking loci to sucrose storage. What is new is the combination of phased assembly with association analysis in a polyploid crop, rather than assembly alone.
Published papers
Genetic architecture of sugarcane traits in a polyploid genomics framework.
Phased POJ2878 sugarcane genome via Pore-C assembly and polyploid-tailored GWAS of 981 accessions linking loci to sucrose storage.
Preprints
First high-quality genome assemblies with chromosome-scale contiguity of Tunisian durum wheat (Triticum turgidum subsp. durum) landraces Chili and Mahmoudi
Chromosome-scale assemblies of Tunisian durum wheat landraces Chili and Mahmoudi from HiFi and Hi-C data, with 14 pseudomolecules each.
Draft genome assembly of the Woolly bottlebrush (Greyia radlkoferi) using Pacbio long-read sequencing technology.
PacBio HiFi draft genome of Greyia radlkoferi: 206 Mb assembly, scaffold N50 10.1 Mb, 17,804 predicted protein-coding genes.
Topic history
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